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首頁> 外文會議>Microfluidics, bioMEMS, and medical microsystems VIII >Portable Microfluidic Cytometer for Whole Blood Cell Analysis
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Portable Microfluidic Cytometer for Whole Blood Cell Analysis

機譯:便攜式微流式細(xì)胞儀用于全血細(xì)胞分析

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Lab-on-a-chip (LOC) systems allow complex laboratory assays to be carried out on a single chip using less time, reagents, and manpower than traditional methods. There are many chips addressing PCR and other DNA assays, but few that address blood cell analysis. Blood analysis, particularly of the cellular component, is highly important in both medical and scientific fields. Traditionally blood samples require a vial of blood, then several processing steps to separate and stain the various components, followed by the preparations for each specific assay to be performed. A LOC system for blood cell analysis and sorting would be ideal. The microfluidic-based system we have developed requires a mere drop of blood to be introduced onto the chip. Once on chip, the blood is mixed with both fluorescent and magnetic labels. The lab-on-a-chip device then uses a syringe drive to push the cells through the chip, while a permanent magnet is positioned to pull the magnetically labeled white blood cells to a separate channel. The white blood cells, labeled with different color fluorescent quantum dots (Qdots) conjugated to antibodies against WBC subpopulations, are analyzed and counted, while a sampling of red blood cells is also counted in a separate channel. This device will be capable of processing whole blood samples on location in a matter of minutes and displaying the cell count and should eventually find use in neonatology, AIDS and remote site applications.
機譯:芯片實驗室(LOC)系統(tǒng)允許在單個芯片上執(zhí)行復(fù)雜的實驗室分析,比傳統(tǒng)方法花費的時間,試劑和人力更少。有許多芯片可用于PCR和其他DNA分析,但很少有芯片可用于血細(xì)胞分析。血液分析,特別是細(xì)胞成分的血液分析,在醫(yī)學(xué)和科學(xué)領(lǐng)域都非常重要。傳統(tǒng)上,血液樣本需要一小瓶血液,然后需要幾個處理步驟來分離和染色各種成分,然后為要執(zhí)行的每個特定測定做準(zhǔn)備。用于血細(xì)胞分析和分類的LOC系統(tǒng)將是理想的。我們開發(fā)的基于微流體的系統(tǒng)僅需將一小滴血液引入芯片即可。一旦進(jìn)入芯片,血液就會與熒光標(biāo)記和磁性標(biāo)記混合。然后,芯片實驗室設(shè)備使用注射器驅(qū)動器將細(xì)胞推過芯片,同時放置永久磁鐵以將磁性標(biāo)記的白細(xì)胞拉至單獨的通道。分析并計數(shù)標(biāo)記有與WBC亞群抗體偶聯(lián)的不同顏色熒光量子點(Qdot)的白細(xì)胞,同時還在單獨的通道中對紅細(xì)胞采樣進(jìn)行計數(shù)。該設(shè)備將能夠在數(shù)分鐘內(nèi)就位處理全血樣本并顯示細(xì)胞計數(shù),最終將在新生兒醫(yī)學(xué),艾滋病和偏遠(yuǎn)地區(qū)應(yīng)用中得到應(yīng)用。

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