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首頁> 外文學(xué)位 >Identification and characterization of genes unique to systemic Xanthomonas pathogens.
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Identification and characterization of genes unique to systemic Xanthomonas pathogens.

機(jī)譯：鑒定和鑒定系統(tǒng)性黃單胞菌病原體特有的基因。

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Suppression Subtractive Hybridization (SSH) was used to identify genes present in the systemic pathogen Xanthomonas campestris pv. campestris (XCC), and missing from the non systemic pathogen Xanthomonas campestris pv. armoraciae (XCA). Among the DNA fragments unique to XCC was Xcc2109, one of eight putative avr genes identified in the XCC528T genome (NC 003902). Individual and sequential deletion and/or insertion mutations of all eight putative XCC avr gene loci were made, but no change in pathogenicity was observed in any combination of avr mutations, including a strain with all eight avr genes deleted. However, insertion or deletion mutants at the Xcc2109 locus lost avirulence (i.e., became virulent) on Brassica juncea, a race-determining differential host. The Xcc2109 ORF as annotated was cloned and found to be nonfunctional. A longer gene, encompassing Xcc2109 and here designated avrXccFM, was defined and found to confer avirulence to Xcc2109 mutant strains and to two additional wild type strains, thereby changing their designation. Resistance in B. juncea to XCC528T strains carrying avrXccFM occurs without a typical HR, but with a vascular HR (VHR).; A similar but modified SSH was performed between the two systemic pathogens Xanthomonas phaseoli (XP) and Xanthomonas axonopodis pv. phaseoli var. fuscans (XAP), and the non systemic Xanthomonas axonopodis pv. alfalfae (XAA). Among other gene fragments, a large number of transposable elements as well as gene fragments of unknown function were obtained. Five genes also present in other bacterial pathogens were mutagenized in XP. None of the mutant strains obtained exhibited any effect in virulence except for a pthA homolog, a gene earlier identified in XP and included in the functional study. Complementation assays were inconclusive although in detached leaves transient expression of the pthA gene homolog obtained from an XPF cosmid library, induced a phenotype similar to common bacterial blight of bean.
機(jī)譯：使用抑制消減雜交（SSH）來鑒定系統(tǒng)性病原體Xanthomonas campestris pv中存在的基因。 campestris（XCC），并從非系統(tǒng)性病原體Xanthomonas campestris pv中丟失。甲蟲（XCA）。 Xcc獨(dú)特的DNA片段中有Xcc2109，它是XCC528T基因組（NC 003902）中鑒定的八個(gè)推定avr基因之一。進(jìn)行了所有八個(gè)推定的XCC avr基因位點(diǎn)的個(gè)體和順序缺失和/或插入突變，但在avr突變的任何組合中都未觀察到致病性變化，包括所有八個(gè)avr基因都缺失的菌株。然而，在Xcc2109基因座處的插入或缺失突變體在決定種族的差異宿主Brucesica juncea上喪失了無毒力（即變得有毒力）。注釋中的Xcc2109 ORF被克隆，發(fā)現(xiàn)沒有功能。定義了一個(gè)更長的基因，包括Xcc2109，在這里命名為avrXccFM，發(fā)現(xiàn)該基因賦予Xcc2109突變株和另外兩個(gè)野生型株無毒力，從而改變了它們的名稱。在沒有典型HR的情況下，芥菜芽孢桿菌對(duì)攜帶avrXccFM的XCC528T菌株產(chǎn)生抗性，但在血管性HR（VHR）下發(fā)生。在兩種系統(tǒng)性病原體黃單胞菌（XP）和軸突黃單胞菌pv之間進(jìn)行了類似但經(jīng)過修改的SSH。菜豆變種fuscans（XAP）和非系統(tǒng)性Xanthomonas axonopodis pv。苜蓿（XAA）。在其他基因片段中，獲得了大量的轉(zhuǎn)座因子以及功能未知的基因片段。 XP中也誘變了其他細(xì)菌病原體中也存在的五個(gè)基因。除了pthA同源物（一種在XP中較早鑒定并包含在功能研究中的基因）外，獲得的所有突變菌株均未顯示出任何毒力。盡管在離體的葉子中瞬時(shí)表達(dá)從XPF粘粒文庫中獲得的pthA基因同源物，誘導(dǎo)的表型類似于豆類常見的枯萎病，但互補(bǔ)性分析尚無定論。

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  • 作者

    Castaneda C., Luz Adriana.;

  • 作者單位

    University of Florida.;

  • 授予單位 University of Florida.;
  • 學(xué)科 Agriculture Plant Pathology.; Biology Molecular.
  • 學(xué)位 Ph.D.
  • 年度 2005
  • 頁碼 135 p.
  • 總頁數(shù) 135
  • 原文格式 PDF
  • 正文語種 eng
  • 中圖分類 植物病理學(xué);分子遺傳學(xué);
  • 關(guān)鍵詞

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