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首頁> 外文學位 >Controlling protein-silicone interactions by the modification of silicone elastomers with poly(ethylene oxide).
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Controlling protein-silicone interactions by the modification of silicone elastomers with poly(ethylene oxide).

機譯：通過用聚環(huán)氧乙烷改性有機硅彈性體來控制蛋白質(zhì)-有機硅相互作用。

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摘要

Despite their special and unique properties, the inherent hydrophobicity of silicone elastomers compromises their use in certain biological applications because interactions with local biology, such as proteins, can be detrimental. It is possible, however, to modify these interactions by tuning the elastomer hydrophobicity through controlling its composition using additives such as poly(ethylene oxide)(PEO), a hydrophilic, protein compatible polymer.; Enzyme entrapment in silicone elastomers serves as a convenient model to study protein silicone interactions because enzyme viability, which is affected by the entrapping matrix composition, serves as an indicator of the rubber biocompatibility. Two enzymes of different nature, lipase C. rugosa and alpha-chymotrypsin were entrapped in silicone rubber, and their activities in the presence and the absence of PEO were examined.; The interaction between the enzymes and the elastomer were found to be dependent on both the nature of the enzyme and the rubber composition. It was found that the interaction between lipase C. rugosa, a lipophilic enzyme, and the entrapping matrix benefits from the hydrophobic nature of the silicone rubber, and leads to active bio-catalytic materials. By contrast, alpha-chymotrypsin was affected negatively by the hydrophobicity of the silicone, and only become stable within the elastomer when PEO was also incorporated within the rubber formulation.; The effects of various factors, including the concentration and chain length of PEO, the enzyme concentration, and the cross-linker concentration on alpha-chymotrypsin activity were studied, following a chemometric approach. Results indicated that the specific activity can be maximized by incorporating a relatively high content of short chain, functional PEO. The enzyme concentration was found to adversely affect the specific activity, while the effect of the crosslinker TEOS was found to be insignificant when PEO was present in the elastomer; however, it did affect the activity positively in the case of simple elastomers.; These results show that it is extremely important to design an elastomer matrix to fit the requirements of a specific protein. With an appropriate balance of hydrophobic and hydrophilic constituents, bioactive silicone elastomers containing either lipophilic (lipase) or hydrophilic (chymotrypsin) enzymes can be prepared.
機譯：盡管有機硅彈性體具有特殊和獨特的性能，但其固有的疏水性卻損害了它們在某些生物學應用中的使用，因為與蛋白質(zhì)等局部生物學的相互作用可能有害。但是，可以通過使用諸如聚環(huán)氧乙烷（PEO）（一種親水性，蛋白質(zhì)相容性聚合物）之類的添加劑控制彈性體的組成來調(diào)節(jié)彈性體的疏水性，從而改變這些相互作用。有機硅彈性體中的酶截留是研究蛋白質(zhì)有機硅相互作用的便捷模型，因為受截留的基質(zhì)組成影響的酶活力是橡膠生物相容性的指標。將兩種性質(zhì)不同的酶，即脂肪酶C.rugosa和α-胰凝乳蛋白酶包埋在硅橡膠中，并檢查了它們在有無PEO的情況下的活性。發(fā)現(xiàn)酶和彈性體之間的相互作用取決于酶和橡膠組合物的性質(zhì)。已經(jīng)發(fā)現(xiàn)，脂肪酶C.rugosa，親脂性酶和截留基質(zhì)之間的相互作用得益于硅橡膠的疏水性，并導致活性的生物催化材料。相反，α-胰凝乳蛋白酶受到硅氧烷的疏水性的負面影響，只有當PEO也摻入橡膠配方中時，α-胰凝乳蛋白酶才在彈性體中變得穩(wěn)定。采用化學計量學方法研究了各種因素，包括PEO的濃度和鏈長，酶的濃度以及交聯(lián)劑的濃度對α-胰凝乳蛋白酶活性的影響。結(jié)果表明，通過摻入較高含量的短鏈功能性PEO，可以使比活最大化。發(fā)現(xiàn)酶濃度對比活性有不利影響，而當彈性體中存在PEO時，交聯(lián)劑TEOS的作用微不足道。但是，對于簡單的彈性體，它確實對活性有積極影響。這些結(jié)果表明，設計適合特定蛋白質(zhì)要求的彈性體基質(zhì)非常重要。通過疏水和親水成分的適當平衡，可以制備含有親脂性（脂肪酶）或親水性（胰凝乳蛋白酶）酶的生物活性有機硅彈性體。

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  • 作者

    Ragheb, Amro M.;

  • 作者單位

    McMaster University (Canada).;

  • 授予單位 McMaster University (Canada).;
  • 學科 Chemistry Polymer.
  • 學位 Ph.D.
  • 年度 2005
  • 頁碼 187 p.
  • 總頁數(shù) 187
  • 原文格式 PDF
  • 正文語種 eng
  • 中圖分類 高分子化學（高聚物）;
  • 關鍵詞

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