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首頁> 外文學(xué)位 >Mapping and confirmation of QTL for resistance to gray leaf spot in perennial ryegrass.
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Mapping and confirmation of QTL for resistance to gray leaf spot in perennial ryegrass.

機譯：多年生黑麥草對灰葉斑病抗性的QTL定位和確認。

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Gray leaf spot (GLS) is a serious disease caused by the fungus Pyricularia grisea, recently reported on the important turfgrass species, perennial ryegrass (Lolium perenne L.). This fungus also causes blast in rice, which is usually controlled by host resistance, though resistance durability is problematic. Most perennial ryegrass cultivars are susceptible to GLS. However, greenhouse inoculations using one ryegrass isolate and one rice-infecting strain revealed partial resistance in an Italian x perennial ryegrass mapping population. A linkage map of this population was used to identify quantitative trait loci (QTL) for GLS resistance. QTL were detected on four linkage groups (LGs), and resistance to the different strains were associated with different QTL. Of three QTL detected using the ryegrass isolate, the one with strongest effect was located on LG3 of the MFB parent, explaining 20%-37% of the phenotypic variance. Another QTL was detected on LG6 of the MFA parent, explaining 5%-10% of the phenotypic variance. For resistance to the rice strain, two QTL were located on LGs MFA-2 and MFB-4, each explaining about 10% of the phenotypic variance. Further, QTL on LGs 3 and 4 appeared syntenic to blast resistance loci in rice.; To confirm that QTL from the MFAxMFB population are still detected in the same locations in the next generation, a resistant segregant from that population was crossed with an unrelated susceptible perennial clone, to form a new mapping population segregating for GLS resistance. QTL analysis was performed, using two different ryegrass field isolates and RAPD, RFLP, and SSR marker-based linkage maps for each parent. Results indicate the QTL on LG3 of the resistant parent is still significant, with LOD and percent of phenotypic variance explained ranging from 2.0 to 4.2 and 6% to 11%, respectively. Two new QTL were detected in the susceptible parent, with similar LOD and phenotypic variance explained. Although the LG6 QTL was not detected, the major QTL on LG3 was confirmed. The ultimate goal of these studies is understanding the genetic architecture of GLS resistance, facilitating its utilization in perennial ryegrass breeding via marker-assisted selection, and benefiting growers of perennial ryegrass.
機譯：灰葉斑?。℅LS）是一種由稻瘟病菌引起的嚴重疾病，最近報道了重要的草皮草物種多年生黑麥草（Lolium perenne L.）。這種真菌還會在稻米中引起稻瘟病，這通常受宿主抗性控制，盡管抗性耐久性存在問題。大多數(shù)多年生黑麥草品種對GLS敏感。但是，在意大利x多年生黑麥草制圖種群中，使用一種黑麥草分離株和一種水稻感染菌株進行的溫室接種顯示出部分抗性。該人群的連鎖圖用于鑒定GLS抗性的數(shù)量性狀基因座（QTL）。在四個連鎖群（LG）上檢測到QTL，并且對不同菌株的抗性與不同的QTL相關(guān)。在使用黑麥草分離株檢測到的三個QTL中，效果最強的一個位于MFB親本的LG3上，解釋了20％-37％的表型變異。在MFA親本的LG6上檢測到另一個QTL，解釋了表型變異的5％-10％。為了對水稻菌株產(chǎn)生抗性，兩個QTL位于LG MFA-2和MFB-4上，每個QTL解釋了約10％的表型變異。另外，LGs 3和4上的QTL與水稻的抗稻瘟病位點呈同義關(guān)系。為了確認仍在下一代的相同位置檢測到MFAxMFB群體的QTL，將該群體的抗性分離劑與無關(guān)的易感多年生克隆雜交，以形成針對GLS耐藥性分離的新作圖群體。使用兩個不同的黑麥草田間分離物以及每個親本的基于RAPD，RFLP和SSR標(biāo)記的連鎖圖譜進行QTL分析。結(jié)果表明，抗性親本的LG3上的QTL仍然很顯著，其LOD和表型變異百分數(shù)解釋為分別在2.0至4.2和6％至11％之間。在易感父母中檢測到兩個新的QTL，并解釋了相似的LOD和表型差異。盡管未檢測到LG6 QTL，但已確認LG3上存在主要QTL。這些研究的最終目標(biāo)是了解GLS抗性的遺傳結(jié)構(gòu)，通過標(biāo)記輔助選擇促進其在多年生黑麥草育種中的利用，并使多年生黑麥草的種植者受益。

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