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首頁> 外文學(xué)位 >Development of high-throughput biosensors for multi-sample determination of biochemical oxygen demand (BOD) based on optical detection of oxygen.
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Development of high-throughput biosensors for multi-sample determination of biochemical oxygen demand (BOD) based on optical detection of oxygen.

機(jī)譯：開發(fā)高通量生物傳感器，用于基于光學(xué)檢測(cè)氧氣的多樣品測(cè)定生化需氧量（BOD）。

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Biochemical oxygen demand (BOD) is one of the most important and frequently used parameters for estimating the level of organic pollutants in water. Microbial BOD sensors have received much attention as they provide fast, portable and cost-effective methods for environmental monitoring and rapid determination of wastewater quality in wastewater treatment plants. In this project, we aim at developing a high-throughput multi-sample BOD sensor for BOD determination of a large number of wastewater samples based on the optical sensing of dissolved oxygen concentration.; Two prototype optical biosensors for multi-sample BOD determination have been constructed based upon the optical sensing of change in dissolved oxygen concentration by the luminescent metal complex Ru[(dpp)3] 2+ (dpp = 4,7-diphenyl-1,10-phenanthroline). In the first prototype BOD sensor, activated sludge and Bacillus subtilis strain 1A304 (&phis;105MU331) were immobilized on oxygen sensing films on the bottom of glass sample vials. This BOD biosensor was found to retain about 77% of its initial activity for activated sludge and 88% for B. subtilis after stored at 4°C for 30 days. Good agreement was achieved between the results of the BOD measurement by the activated sludge biosensor and those obtained from conventional BOD5 method for water samples. However, only fairly good agreement was obtained by the B. subtilis biosensor. The results show that the B. subtilis biosensor may be suitable for wastewater with low BOD levels, as the microbes cannot assimilate a wide enough range of organic substrates.; The second prototype BOD biosensor was constructed using ormosil oxygen sensing films and Stenotrophomonas maltophilia immobilized in an ormosil polymer hybrid material of tetramethoxysilane (TMOS), methyltrimethoxysilane (MTMS) and poly(vinyl alcohol) (PVA).{09}This BOD biosensor has a wide range of BOD loading (linearly up to about 60 mg l-1 GGA standard), longer lifetime, higher stability and better correlation of low BOD5 values (especially for GGA solutions) as compared to the first prototype biosensor. Good agreement was achieved between the results of the BOD measurement by the immobilized Stenotrophomonas maltophilia biosensor and those obtained from conventional BOD5 method for water samples. The particular strain Stenotrophomonas maltophilia isolated from activated sludge is shown to be suitable for BOD biosensor fabrication. (Abstract shortened by UMI.)
機(jī)譯：生化需氧量（BOD）是估算水中有機(jī)污染物含量的最重要且最常用的參數(shù)之一。微生物BOD傳感器已受到廣泛關(guān)注，因?yàn)樗鼈優(yōu)榄h(huán)境監(jiān)測(cè)和廢水處理廠廢水質(zhì)量的快速確定提供了快速，便攜式和經(jīng)濟(jì)高效的方法。在這個(gè)項(xiàng)目中，我們的目標(biāo)是開發(fā)一種高通量的多樣品BOD傳感器，該傳感器基于溶解氧濃度的光學(xué)傳感，用于BOD測(cè)定大量廢水樣品?；诎l(fā)光金屬絡(luò)合物Ru [（dpp）3] 2+（dpp = 4,7-diphenyl-1,10,10）的溶解氧濃度變化的光學(xué)傳感，已構(gòu)建了兩個(gè)用于多樣品BOD測(cè)定的原型光學(xué)生物傳感器。 -菲咯啉）。在第一個(gè)原型BOD傳感器中，將活性污泥和枯草芽孢桿菌菌株1A304（φ105MU331）固定在玻璃樣品瓶底部的氧氣感應(yīng)膜上。發(fā)現(xiàn)該BOD生物傳感器在4°C下保存30天后，對(duì)活性污泥保留約77％的初始活性，對(duì)枯草芽孢桿菌保留88％的初始活性?；钚晕勰嗌飩鞲衅鲗?duì)BOD的測(cè)量結(jié)果與從常規(guī)BOD5方法獲得的用于水樣的結(jié)果之間達(dá)成了良好的一致性。然而，枯草芽孢桿菌生物傳感器僅獲得了相當(dāng)好的協(xié)議。結(jié)果表明，枯草芽孢桿菌生物傳感器可能適用于低BOD水平的廢水，因?yàn)槲⑸锊荒芪兆銐驈V泛的有機(jī)底物。第二個(gè)原型BOD生物傳感器是使用ormosil氧感測(cè)膜和嗜麥芽窄食單胞菌固定在由四甲氧基硅烷（TMOS），甲基三甲氧基硅烷（MTMS）和聚乙烯醇（PVA）構(gòu)成的ormosil聚合物雜化材料中的。{09}與第一個(gè)原型生物傳感器相比，寬范圍的BOD負(fù)載（線性高達(dá)約60 mg l-1 GGA標(biāo)準(zhǔn)品），更長(zhǎng)的壽命，更高的穩(wěn)定性以及低BOD5值之間的相關(guān)性更好（尤其是對(duì)于GGA解決方案）。固定化嗜麥芽窄食單胞菌生物傳感器的生化需氧量測(cè)量結(jié)果與通過水樣品的常規(guī)生化需氧量方法獲得的結(jié)果之間達(dá)成了良好的一致性。從活性污泥中分離出的嗜麥芽單胞菌嗜麥芽孢桿菌特別菌株適合于BOD生物傳感器的制造。 （摘要由UMI縮短。）

著錄項(xiàng)

  • 作者

    Kwok, Nga Yan.;

  • 作者單位

    Hong Kong Polytechnic University (People's Republic of China).;

  • 授予單位 Hong Kong Polytechnic University (People's Republic of China).;
  • 學(xué)科 Engineering Sanitary and Municipal.; Engineering Environmental.
  • 學(xué)位 Ph.D.
  • 年度 2005
  • 頁碼 315 p.
  • 總頁數(shù) 315
  • 原文格式 PDF
  • 正文語種 eng
  • 中圖分類 建筑科學(xué);環(huán)境污染及其防治;
  • 關(guān)鍵詞

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