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首頁> 外文學(xué)位 >Antiviral activities of selected Hong Kong marine algae against herpes simplex viruses and other viruses and their possible antiviral mechanisms.
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Antiviral activities of selected Hong Kong marine algae against herpes simplex viruses and other viruses and their possible antiviral mechanisms.

機(jī)譯：某些香港海藻對(duì)單純皰疹病毒和其他病毒的抗病毒活性及其可能的抗病毒機(jī)制。

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摘要

Initially, aqueous extracts from eight species of Hong Kong marine algae were screened for their antiviral activity against herpes simplex viruses type 1 (HSV-1) and type 2 (HSV-2) by cytopathogenic effect (CPE) inhibition assay. Two kinds of the extracts were found to have potent anti-HSV activities. An anti-HSV polysaccharide fraction (Fr2a) was isolated from the brown alga Sargassum patens by bioactivity-guided fractionation. The analysis on the chemical and physical characterizations of Fr2a demonstrated that the active compound is a sulfated polysaccharide with molecular weight of 424 kDa.; Fr2a exhibited very low cellular toxicity to Vero cells with CC 50 greater than 2,500 μg/ml by MTT assay and LDH assay. The EC 50 values of Fr2a against HSV-2 standard strain (8702) and clinical strain were 1.3 and 1.65 μg/ml respectively by plaque reduction assay. The selective index of Fr2a against HSV-2 was estimated to be higher than 1923. Fr2a also significantly inhibited the replication of HSV-1 acyclovir resistant strain and sensitive strain in vitro in a dose-dependent manner with EC50 of 1.5 to 5.3 μg/ml.; Treatment of host cells with Fr2a before infection showed no inhibitory effect against HSV-2 replication. Fr2a did not protect the cells from virus infection. But Fr2a exhibited an extracellular virucidal effect against HSV-2 in high concentration with EC50 of 50 μg/ml. In low concentration, Fr2a could not inactivate the viral particles. The virus inactivation by Fr2a was temperature-dependent and time-independent.; The pretreatment of host cells with Fr2a for 24 h could not cause inhibition against HSV-1 in viral yield. Fr2a had weak virucidal activity against HSV-1 ACV sensitive strain at the concentration of 100 μg/ml. However, it did not show any virucidal activity against HSV-1 acyclovir resistant strain at this concentration. In viral replication cycle of HSV-1 assay, Fr2a was found not to decrease the virus yield of ACV resistant strain when it was added after 1 h adsorption. However, it could inhibit ACV sensitive strain replication to some extent. (Abstract shortened by UMI.)
機(jī)譯：最初，通過細(xì)胞致病作用（CPE）抑制試驗(yàn)篩選了八種香港海藻的水提物對(duì)1型單純皰疹病毒（HSV-1）和2型單純皰疹病毒（HSV-2）的抗病毒活性。發(fā)現(xiàn)兩種提取物具有有效的抗HSV活性。通過生物活性指導(dǎo)分離從褐藻中分離出抗HSV多糖組分（Fr2a）。對(duì)Fr2a的化學(xué)和物理特性的分析表明，該活性化合物是分子量為424kDa的硫酸化多糖。通過MTT法和LDH法檢測(cè)，F(xiàn)r2a對(duì)Vero細(xì)胞的細(xì)胞毒性非常低，CC 50 大于2,500μg/ ml。通過噬斑減少試驗(yàn)，F(xiàn)r2a對(duì)HSV-2標(biāo)準(zhǔn)株（8702）和臨床株的EC 50 值分別為1.3和1.65μg/ ml。據(jù)估計(jì)，F(xiàn)r2a對(duì)HSV-2的選擇性指數(shù)高于1923。Fr2a還以EC 50 1.5至5.3μg/ ml;感染前用Fr2a處理宿主細(xì)胞對(duì)HSV-2復(fù)制沒有抑制作用。 Fr2a不能保護(hù)細(xì)胞免受病毒感染。但是，F(xiàn)r2a對(duì)ECV 50 為50μg/ ml的高濃度HSV-2具有殺滅細(xì)胞外的作用。在低濃度下，F(xiàn)r2a不能滅活病毒顆粒。 Fr2a對(duì)病毒的滅活是溫度依賴性和時(shí)間依賴性的。用Fr2a預(yù)處理宿主細(xì)胞24小時(shí)不會(huì)導(dǎo)致對(duì)HSV-1病毒產(chǎn)量的抑制。在100μg/ ml的濃度下，F(xiàn)r2a對(duì)HSV-1 ACV敏感菌株的殺病毒活性較弱。但是，在此濃度下，它對(duì)HSV-1阿昔洛韋抗性菌株沒有任何殺滅病毒的活性。在HSV-1分析的病毒復(fù)制周期中，發(fā)現(xiàn)Fr2a在吸附1小時(shí)后添加時(shí)不會(huì)降低ACV抗性菌株的病毒產(chǎn)量。但是，它可以在一定程度上抑制ACV敏感菌株的復(fù)制。 （摘要由UMI縮短。）

著錄項(xiàng)

  • 作者

    Zhu, Wen.;

  • 作者單位

    Chinese University of Hong Kong (People's Republic of China).;

  • 授予單位 Chinese University of Hong Kong (People's Republic of China).;
  • 學(xué)科 Biology Microbiology.
  • 學(xué)位 Ph.D.
  • 年度 2002
  • 頁碼 p.2201
  • 總頁數(shù) 279
  • 原文格式 PDF
  • 正文語種 eng
  • 中圖分類 微生物學(xué);
  • 關(guān)鍵詞

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