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首頁> 外文學(xué)位 >STUDIES ON THE REPRODUCTIVE BIOLOGY OF MALE-STERILE MUTANTS OF SOYBEAN (GLYCINE MAX (L.) MERR.).
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STUDIES ON THE REPRODUCTIVE BIOLOGY OF MALE-STERILE MUTANTS OF SOYBEAN (GLYCINE MAX (L.) MERR.).

機(jī)譯:大豆雄性不育突變體的生殖生物學(xué)研究(Glycine max(L.)MERR。)。

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摘要

The phenotypic effects of three nuclear male-sterile mutants on reproductive biology in soybean (Glycine max (L.) Merr.) were observed by means of light and electron microscopy. The ms2 mutant interrupted microsporogenesis at the tetrad stage. Postmeiotic cytokinesis deliminated tetrads of microspores. Callose dissolution did not occur, and microspores degenerated after depositing primexine and probacullae. Male sterility was associated with, and perhaps induced by, aberrant ontogeny of the tapetal layer.; The ms2 mutant also was shown to have a pleiotropic effect on female reproduction. Spontaneous trisomy occurred among progeny at a rate of 2.4%. Aneuploid lines isolated were evaluated for their stability and potential usefulness in the construction of a trisomic series in soybean.; The ms4 mutant was shown to inhibit, or render abnormal, developmental processes related to the function of the postmeiotic MMC plasmalemma. The nature of postmeiotic cytokinesis was variable. Frequently, cytokinesis was omitted, or incomplete and/or irregular in orientation. Less often, cytokinesis was normal in orientation, which led to, but did not ensure, the formation of normal pollen. Postmeiotic (PM) cells varied in the degree of pollen wall organization achieved. Pollen-like walls ranged from only random and scattered sporopollenin deposits to stratified walls indistinguishable from normal pollen walls. Plants homozygous for the ms4 allele were able to generate pollen with features identical to those of normal pollen. Thus, the ms4 mutant was considered a partial male-sterile mutant.; A spontaneous male-sterile mutant that arose in 'Wabash' soybean was analyzed both genetically and cytologically. The "Wabash male-sterile" mutant was shown to be an independent mutation at the ms3 locus. Despite diverse nuclear backgrounds, the Wabash ms3 (Wms3) and the 'Calland' x 'Cutler' (original) ms3 were characterized by similar phenotypes. Microspores were generated but produced abnormal walls and were not released from callose. Tapetal cells either degenerated prematurely, or accumulated a refractive material that was thought to be sporopollenin.
機(jī)譯:通過光鏡和電子顯微鏡觀察了三個核不育突變體對大豆生殖生物學(xué)的表型效應(yīng)(Glycine max(L.)Merr。)。在第四個階段,ms2突變體中斷了微孢子發(fā)生。減數(shù)分裂后的胞質(zhì)分裂消除了小孢子的四分體。沒有發(fā)生ose的溶解,并且沉積了primemine和probacullae后小孢子變性了。雄性不育與絨氈層異常的個體發(fā)育有關(guān),也可能是由其引起的。還顯示了ms2突變體對女性生殖有多效作用。自發(fā)三體性發(fā)生在子代中,發(fā)生率為2.4%。評價分離的非整倍體品系的穩(wěn)定性和在構(gòu)建大豆三體系列中的潛在用途。已證明ms4突變體可抑制減數(shù)分裂后MMC血漿障礙功能或使其異常發(fā)育。減數(shù)分裂后胞質(zhì)分裂的性質(zhì)是可變的。通常,省略胞質(zhì)分裂,或取向不完全和/或不規(guī)則。較少的胞質(zhì)分裂定向是正常的,這導(dǎo)致但不能確保正常的花粉形成。減數(shù)分裂后(PM)細(xì)胞的花粉壁組織程度不同?;ǚ蹣颖诘姆秶鷱膬H隨機(jī)的和分散的孢粉蛋白沉積到與正常花粉壁無法區(qū)分的分層壁。 ms4等位基因純合的植物能夠產(chǎn)生具有與正常花粉相同特征的花粉。因此,ms4突變體被認(rèn)為是部分雄性不育突變體。從遺傳和細(xì)胞學(xué)上分析了自“ Wabash”大豆中產(chǎn)生的自發(fā)雄性不育突變體。顯示“ Wabash雄性不育”突變體是ms3位點(diǎn)的獨(dú)立突變。盡管有不同的核背景,沃巴什(Wabash)ms3(Wms3)和“ Calland” x“ Cutler”(原始)ms3的特征也相似。產(chǎn)生了小孢子,但是產(chǎn)生了異常的壁,并且沒有從call中釋放出來。絨氈膜細(xì)胞要么過早變性,要么積累了一種被認(rèn)為是孢粉的折射物質(zhì)。

著錄項

  • 作者

    GRAYBOSCH, ROBERT ALLEN.;

  • 作者單位

    Iowa State University.;

  • 授予單位 Iowa State University.;
  • 學(xué)科 Biology Genetics.
  • 學(xué)位 Ph.D.
  • 年度 1984
  • 頁碼 172 p.
  • 總頁數(shù) 172
  • 原文格式 PDF
  • 正文語種 eng
  • 中圖分類 遺傳學(xué);
  • 關(guān)鍵詞

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