Aim: Shigella infection is an important global health problem in developing c...">

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Invasion of HEp-2 cells by Shigella spp. isolated from acute pediatric diarrhea

機譯：志賀氏菌對HEp-2細(xì)胞的侵襲。分離自急性小兒腹瀉

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>Aim: Shigella infection is an important global health problem in developing countries where hygiene is poor and hence shigellosis is a main cause of diarrhoea-associated mortality and morbidity, particularly in children under the age of five. The bacterial entry into colon and rectal epithelial cells has been named ‘bacterium-directed phagocytosis’. This term highlights that the bacteria actively stimulate their own uptake into non-professional phagocytes. The aim of this study was to demonstrate the invasion of HEp-2 cells by Shigella spp. isolated from acute pediatric diarrhea in Tehran, Iran.>Methods: Three-hundred and ten non-duplicative diarrheal stool samples were collected from the children admitted to Children’s Medical Center in Tehran, Iran. Samples were cultured and suspected colonies were identified by routine microbiological and biochemical tests. The invasion of the two isolated Shigella spp. to HEp-2 cells was studied.>Results: Of 310 stool samples, 16 (5.2%) Shigella spp. were isolated, including seven (43.7%) S. sonnei and nine (56.3%) S. flexneri. Four (44.4%) S. sonnei and seven (42.8%) S. flexneri showed invasive phenotype to HEp-2.>Conclusion: Shigella sonnei and S. flexneri are reported as the most prevalent Shigella spp. in nature which infect humans. Invasion of various cell lines gives the chance of survival to Shigella spp. This ability causes more virulent infections in the host. Despite costly and time consuming cell culture techniques, the current method described in this paper is reliable for detecting invasive behavior of Shigella spp. Results have also shown that not all the Shigella spp. are able to invade intestinal epithelial cells.
機譯：>目標(biāo)：志賀氏菌感染是發(fā)展中國家的一個重要的全球性健康問題，這些國家的衛(wèi)生狀況較差，因此志賀菌病是引起腹瀉相關(guān)死亡率和發(fā)病率的主要原因，尤其是在五歲以下的兒童中。細(xì)菌進入結(jié)腸和直腸上皮細(xì)胞的過程被稱為“細(xì)菌定向吞噬作用”。該術(shù)語突出表明細(xì)菌會主動刺激自身吸收到非專業(yè)吞噬細(xì)胞中。這項研究的目的是證明志賀氏菌對HEp-2細(xì)胞的侵襲。從伊朗德黑蘭的急性小兒腹瀉中分離出來。>方法：從伊朗德黑蘭兒童醫(yī)學(xué)中心收治的兒童中收集了300和10個非重復(fù)性腹瀉糞便樣本。培養(yǎng)樣品并通過常規(guī)的微生物學(xué)和生化測試鑒定可疑菌落。兩個孤立的志賀氏菌屬的入侵。 >結(jié)果：在310個糞便樣本中，有16個（5.2％）志賀氏菌屬。分離出的包括7（43.7％）的S. sonnei和9（56.3％）的S. flexneri。四個（44.4％）的S. sonnei和七個（42.8％）的S. flexneri對HEp-2表現(xiàn)出侵襲性表型。在自然界中感染人類。各種細(xì)胞系的侵襲使志賀氏菌得以存活。這種能力在宿主中引起更多的強毒感染。盡管昂貴且費時的細(xì)胞培養(yǎng)技術(shù)，本文描述的當(dāng)前方法對于檢測志賀氏菌的入侵行為還是可靠的。結(jié)果還表明，并非所有志賀氏菌屬。能夠侵襲腸上皮細(xì)胞。

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