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Evaluation of a new combined Western and line blot assay (EUROLINE-WB) for diagnosis and species identification of Echinococcus infection in humans

機(jī)譯：評(píng)價(jià)新的結(jié)合蛋白質(zhì)印跡法和線印跡法（EUROLINE-WB）的診斷和鑒定人類(lèi)棘球infection病

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Serological detection of echinococcosis is crucial for diagnosis and management. We evaluated the new blot assay Euroline-WB (ELB, Euroimmun) which consists of a Western blot with Echinococcus multilocularis (E.m.) vesicle antigens and a line blot part with recombinant antigens from E. granulosus (E.g., genus-specific EgAgB) and E.m. (species-specific Em18 and Em95), in comparison to a commercial Western Blot (EWB, LDBio) for detection and species differentiation of echinococcosis within routine laboratory diagnostics. Thirty-five serum samples from 35 patients classified according to a standardized classification were included in the analysis. Out of 24 cases of proven and probable infection with E.m. or E.g. 16 (66.7%) and 15 (62.5%) were correctly identified on species level by EWB and ELB, respectively. False Echinococcus species were assigned in two cases by EWB but none by ELB. Negative blot results in patients with proven infections were noticed in 8.3% (ELB) compared to 4.2% (EWB), but were limited to patients with antiparasitic therapy or post-surgery indicating a treatment-induced loss of antibody activity. Thus, identification of Echinococcus infection at least on the genus level was possible in 23/24 (95.8%) and 19/24 (79.2%) of patients by EWB and ELB (or 22/24 patients (91.7%) including borderline results of ELB), respectively. Recombinant Em18 and Em95 were highly specific for detection of E.m. infection but differed in sensitivity (Em18 56% and 80 %, and Em95 22% and 20% in proven and probable infections, respectively). Advantages of ELB are the standardized analysis of the banding pattern by EUROLineScan software and a faster turn-around-time.
機(jī)譯：血清球菌病的檢測(cè)對(duì)于診斷和管理至關(guān)重要。我們?cè)u(píng)估了新的印跡分析Euroline-WB（ELB，Euroimmun），該印跡分析由具有多棘球chin蟲(chóng)（E.m.）囊泡抗原的Western印跡和具有來(lái)自顆粒腸球菌（E.granususus）（例如屬特異性EgAgB）和E.m. （針對(duì)特定物種的Em18和Em95），與用于在常規(guī)實(shí)驗(yàn)室診斷中檢測(cè)和鑒定棘球and蟲(chóng)病的商業(yè)Western Blot（EWB，LDBio）相比。分析中包括了來(lái)自35名按照標(biāo)準(zhǔn)分類(lèi)法分類(lèi)的35例患者的血清樣本。在確診和可能感染E.m的24例病例中或EWB和ELB分別在物種水平上正確鑒定出16種（66.7％）和15種（62.5％）。 EWB分配了兩種錯(cuò)誤的棘球oc菌種，ELB則沒(méi)有。已證實(shí)感染確診患者的陰性印跡結(jié)果為8.3％（ELB），而4.2％（EWB），但僅限于接受抗寄生蟲(chóng)治療或手術(shù)后的患者，表明治療引起的抗體活性降低。因此，有23.24（95.8％）和19/24（79.2％）的EWB和ELB患者（或22/24患者（91.7％））至少在屬水平上鑒定了埃奇球菌感染是可能的，包括ELB）。重組Em18和Em95對(duì)檢測(cè)E.m具有高度特異性。感染，但敏感性有所不同（Em18已證實(shí)和可能感染分別為56％和80％，Em95為22％和20％）。 ELB的優(yōu)點(diǎn)是可以通過(guò)EUROLineScan軟件對(duì)條帶圖進(jìn)行標(biāo)準(zhǔn)化分析，并且可以縮短周轉(zhuǎn)??時(shí)間。

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