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Microflow Cytometers with Integrated Hydrodynamic Focusing

機譯:集成流體動力聚焦的微流細胞儀

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摘要

This study demonstrates the suitability of microfluidic structures for high throughput blood cell analysis. The microfluidic chips exploit fully integrated hydrodynamic focusing based on two different concepts: Two-stage cascade focusing and spin focusing (vortex) principle. The sample—A suspension of micro particles or blood cells—is injected into a sheath fluid streaming at a substantially higher flow rate, which assures positioning of the particles in the center of the flow channel. Particle velocities of a few m/s are achieved as required for high throughput blood cell analysis. The stability of hydrodynamic particle positioning was evaluated by measuring the pulse heights distributions of fluorescence signals from calibration beads. Quantitative assessment based on coefficient of variation for the fluorescence intensity distributions resulted in a value of about 3% determined for the micro-device exploiting cascade hydrodynamic focusing. For the spin focusing approach similar values were achieved for sample flow rates being 1.5 times lower. Our results indicate that the performances of both variants of hydrodynamic focusing suit for blood cell differentiation and counting. The potential of the micro flow cytometer is demonstrated by detecting immunologically labeled CD3 positive and CD4 positive T-lymphocytes in blood.
機譯:這項研究證明了微流體結(jié)構(gòu)適用于高通量血細胞分析。微流體芯片利用基于兩個不同概念的完全集成的流體動力聚焦:兩級級聯(lián)聚焦和自旋聚焦(渦旋)原理。將樣品(微?;蜓毎膽腋∫海┳⑷胍燥@著較高的流速流動的鞘液中,以確保顆粒位于流動通道的中心。根據(jù)高通量血細胞分析的要求,可以達到幾m / s的粒子速度。通過測量來自校準(zhǔn)珠的熒光信號的脈沖高度分布來評估流體動力粒子定位的穩(wěn)定性?;谧儺愊禂?shù)的熒光強度分布的定量評估得出的微器件利用級聯(lián)水動力聚焦的結(jié)果約為3%。對于自旋聚焦方法,在樣品流速低1.5倍時獲得了相似的值。我們的結(jié)果表明,流體動力學(xué)聚焦的兩個變體的性能適合于血細胞分化和計數(shù)。通過檢測血液中免疫標(biāo)記的CD3陽性和CD4陽性T淋巴細胞證明了微流式細胞儀的潛力。

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