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首頁> 美國衛(wèi)生研究院文獻(xiàn)>ISRN Toxicology >Nitric Oxide Synthase Gene Transfer Overcomes the Inhibition of Wound Healing by Sulfur Mustard in a Human Keratinocyte In Vitro Model
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Nitric Oxide Synthase Gene Transfer Overcomes the Inhibition of Wound Healing by Sulfur Mustard in a Human Keratinocyte In Vitro Model

機(jī)譯：一氧化氮合酶基因轉(zhuǎn)移克服了人角質(zhì)形成細(xì)胞體外模型中硫芥末對傷口愈合的抑制作用。

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Sulfur mustard (SM) is a chemical warfare agent that causes extensive skin injury. Previously we reported that SM exposure resulted in suppression of inducible nitric oxide synthase (iNOS) expression to inhibit the healing of scratch wounds in a cultured normal human epidermal keratinocyte (NHEK) model. Based on this finding, the present study was to use adenovirus-mediated gene transfer of iNOS to restore the nitric oxide (NO) supply depleted by exposure to SM and to evaluate the effect of NO on wound healing inhibited by SM in NHEKs. The effect of the iNOS gene transfer on iNOS protein expression and NO generation were monitored by Western blot and flow cytometry, respectively. Wound healing with or without the iNOS gene transfer after SM exposure was assessed by light and confocal microscopy. The iNOS gene transfer via adenovirus resulted in overexpression of the iNOS and an increase in NO production regardless of SM exposure in the NHEK model. The gene transfer was also effective in overcoming the inhibition of wound healing due to SM exposure leading to the promotion of wound closure. The findings in this study suggest that the iNOS gene transfer is a promising therapeutic strategy for SM-induced skin injury.
機(jī)譯：芥菜（SM）是一種化學(xué)戰(zhàn)劑，會引起廣泛的皮膚傷害。以前我們報道過，在培養(yǎng)的正常人表皮角質(zhì)形成細(xì)胞（NHEK）模型中，SM暴露會導(dǎo)致誘導(dǎo)型一氧化氮合酶（iNOS）表達(dá)受到抑制，從而抑制劃痕傷口的愈合?；诖税l(fā)現(xiàn)，本研究將利用腺病毒介導(dǎo)的iNOS基因轉(zhuǎn)移來恢復(fù)因暴露于SM而耗盡的一氧化氮（NO）供應(yīng)，并評估NO對SM抑制NHEKs傷口愈合的作用。通過蛋白質(zhì)印跡和流式細(xì)胞術(shù)分別監(jiān)測iNOS基因轉(zhuǎn)移對iNOS蛋白表達(dá)和NO生成的影響。通過光鏡和共聚焦顯微鏡評估SM暴露后有無iNOS基因轉(zhuǎn)移的傷口愈合情況。不管是在NHEK模型中暴露于SM還是通過腺病毒進(jìn)行的iNOS基因轉(zhuǎn)移，都導(dǎo)致iNOS的過表達(dá)和NO產(chǎn)生的增加。該基因轉(zhuǎn)移還有效克服了由于暴露于SM而導(dǎo)致傷口閉合促進(jìn)的傷口愈合的抑制。這項(xiàng)研究中的發(fā)現(xiàn)表明，iNOS基因轉(zhuǎn)移是SM誘導(dǎo)的皮膚損傷的有前途的治療策略。

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