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首頁> 美國(guó)衛(wèi)生研究院文獻(xiàn)>Biotechnology Research International >Optimization of Crude Oil and PAHs Degradation by Stenotrophomonas rhizophila KX082814 Strain through Response Surface Methodology Using Box-Behnken Design
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Optimization of Crude Oil and PAHs Degradation by Stenotrophomonas rhizophila KX082814 Strain through Response Surface Methodology Using Box-Behnken Design

機(jī)譯：應(yīng)用Box-Behnken設(shè)計(jì)的響應(yīng)面法優(yōu)化嗜麥芽單胞菌KX082814菌株的原油和PAHs降解

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The present paper describes the process optimization study for crude oil degradation which is a continuation of our earlier work on hydrocarbon degradation study of the isolate Stenotrophomonas rhizophila (PM-1) with GenBank accession number . Response Surface Methodology with Box-Behnken Design was used to optimize the process wherein temperature, pH, salinity, and inoculum size (at three levels) were used as independent variables and Total Petroleum Hydrocarbon, Biological Oxygen Demand, and Chemical Oxygen Demand of crude oil and PAHs as dependent variables (response). The statistical analysis, via ANOVA, showed coefficient of determination R 2 as 0.7678 with statistically significant P value 0.0163 fitting in second-order quadratic regression model for crude oil removal. The predicted optimum parameters, namely, temperature, pH, salinity, and inoculum size, were found to be 32.5°C, 9, 12.5, and 12.5 mL, respectively. At this optimum condition, the observed and predicted PAHs and crude oil removal were found to be 71.82% and 79.53% in validation experiments, respectively. The % TPH results correlate with GC/MS studies, BOD, COD, and TPC. The validation of numerical optimization was done through GC/MS studies and % removal of crude oil.
機(jī)譯：本文介紹了原油降解的工藝優(yōu)化研究，這是我們先前對(duì)GenBank登錄號(hào)分離的嗜麥芽單胞菌（PM-1）分離株嗜烴的研究的延續(xù)。采用Box-Behnken設(shè)計(jì)的響應(yīng)面方法優(yōu)化了溫度，pH，鹽度和接種量（三個(gè)水平）作為自變量，原油的總石油烴，生物需氧量和化學(xué)需氧量的過程和PAHs作為因變量（響應(yīng)）。通過ANOVA進(jìn)行的統(tǒng)計(jì)分析顯示，確定系數(shù)R 2 為0.7678，具有統(tǒng)計(jì)學(xué)意義的P值0.0163擬合二階二次回歸模型進(jìn)行原油去除。預(yù)測(cè)的最佳參數(shù)，即溫度，pH，鹽度和接種量分別為32.5°C，9、12.5和12.5 mL。在此最佳條件下，在驗(yàn)證實(shí)驗(yàn)中發(fā)現(xiàn)和預(yù)測(cè)的PAHs和原油去除率分別為71.82％和79.53％。 TPH的百分比結(jié)果與GC / MS研究，BOD，COD和TPC相關(guān)。數(shù)值優(yōu)化的驗(yàn)證是通過GC / MS研究和％原油去除率完成的。

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