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首頁> 美國衛(wèi)生研究院文獻>Biochemistry Insights >Identification of Stevioside Using Tissue Culture-Derived Stevia (Stevia rebaudiana) Leaves
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Identification of Stevioside Using Tissue Culture-Derived Stevia (Stevia rebaudiana) Leaves

機譯:使用組織培養(yǎng)衍生的甜葉菊(Stevia rebaudiana)葉鑒定甜菊糖苷

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摘要

Stevioside is a natural sweetener from Stevia leaf, which is 300 times sweeter than sugar. It helps to reduce blood sugar levels dramatically and thus can be of benefit to diabetic people. Tissue culture is a very potential modern technology that can be used in large-scale disease-free stevia production throughout the year. We successfully produced stevia plant through in vitro culture for identification of stevioside in this experiment. The present study describes a potential method for identification of stevioside from tissue culture-derived stevia leaf. Stevioside in the sample was identified using HPLC by measuring the retention time. The percentage of stevioside content in the leaf samples was found to be 9.6%. This identification method can be used for commercial production and industrialization of stevia through in vitro culture across the world.
機譯:甜菊糖是甜葉菊的天然甜味劑,甜度是糖的300倍。它有助于顯著降低血糖水平,因此對糖尿病人有益。組織培養(yǎng)是一種非常有潛力的現(xiàn)代技術,可全年用于大規(guī)模無病甜菊生產。我們通過體外培養(yǎng)成功生產了甜葉菊植物,用于在本實驗中鑒定甜菊糖苷。本研究描述了從組織培養(yǎng)衍生的甜葉菊葉中鑒定甜菊糖苷的潛在方法。使用HPLC通過測量保留時間來鑒定樣品中的甜菊糖苷。發(fā)現(xiàn)葉樣品中甜菊糖苷含量的百分比為9.6%。通過全世界的體外培養(yǎng),這種鑒定方法可用于甜菊的商業(yè)化生產和工業(yè)化。

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