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首頁> 美國衛(wèi)生研究院文獻(xiàn)>Biochemical Journal >Were inefficient mitochondrial haplogroups selected during migrations of modern humans? A test using modular kinetic analysis of coupling in mitochondria from cybrid cell lines
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Were inefficient mitochondrial haplogroups selected during migrations of modern humans? A test using modular kinetic analysis of coupling in mitochondria from cybrid cell lines

機譯:在現(xiàn)代人類遷徙過程中是否選擇了效率低下的線粒體單倍群?利用模塊化動力學(xué)分析法對混合細(xì)胞系線粒體進(jìn)行偶聯(lián)的測試

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摘要

We introduce a general test of the bioenergetic importance of mtDNA (mitochondrial DNA) variants: modular kinetic analysis of oxidative phosphorylation in mitochondria from cybrid cells with constant nuclear DNA but different mtDNA. We have applied this test to the hypothesis [Ruiz-Pesini, Mishmar, Brandon, Procaccio and Wallace (2004) Science >303, 223–226] that particular mtDNA haplogroups (specific combinations of polymorphisms) that cause lowered coupling efficiency, leading to generation of less ATP and more heat, were positively selected during radiations of modern humans into colder climates. Contrary to the predictions of this hypothesis, mitochondria from Arctic haplogroups had similar or even greater coupling efficiency than mitochondria from tropical haplogroups.
機譯:我們介紹了mtDNA(線粒體DNA)變體的生物能源重要性的一般測試:線粒體中具有恒定核DNA但不同mtDNA的線粒體中氧化磷酸化的模塊化動力學(xué)分析。我們將此檢驗應(yīng)用于假設(shè)[ruiz-Pesini,Mishmar,Brandon,Procaccio和Wallace(2004)Science > 303 ,223-226]的假設(shè)中,特定的mtDNA單倍群(多態(tài)性的特定組合)導(dǎo)致了降低在現(xiàn)代人向寒冷的氣候輻射期間,積極選擇了耦合效率,導(dǎo)致產(chǎn)生更少的ATP和更多的熱量。與該假設(shè)的預(yù)測相反,北極單倍體的線粒體與熱帶單倍體的線粒體具有相似甚至更高的耦合效率。

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