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Culturable halophilic archaea at the initial and crystallization stages of salt production in a natural solar saltern of Goa India

機(jī)譯：印度果阿的天然太陽能鹽場中在鹽生產(chǎn)的初始階段和結(jié)晶階段可培養(yǎng)的嗜鹽古菌

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BackgroundGoa is a coastal state in India and salt making is being practiced for many years. This investigation aimed in determining the culturable haloarchaeal diversity during two different phases of salt production in a natural solar saltern of Ribandar, Goa. Water and sediment samples were collected from the saltern during pre-salt harvesting phase and salt harvesting phase. Salinity and pH of the sampling site was determined. Isolates were obtained by plating of the samples on complex and synthetic haloarchaeal media. Morphology of the isolates was determined using Gram staining and electron microscopy. Response of cells to distilled water was studied spectrophotometrically at 600nm. Molecular identification of the isolates was performed by sequencing the 16S rRNA.
機(jī)譯：背景果阿是印度的一個(gè)沿海國家，制鹽業(yè)已實(shí)踐多年。這項(xiàng)研究旨在確定果阿里班達(dá)的天然太陽能鹽廠在制鹽的兩個(gè)不同階段中可培養(yǎng)的鹽生古生物多樣性。在預(yù)鹽收獲階段和鹽收獲階段，從鹽廠收集水和沉積物樣品。確定了采樣部位的鹽度和pH。通過將樣品鋪板在復(fù)雜的和合成的鹵代古細(xì)菌培養(yǎng)基上獲得分離物。使用革蘭氏染色和電子顯微鏡確定分離物的形態(tài)。用分光光度法在600nm研究細(xì)胞對蒸餾水的反應(yīng)。通過對16S rRNA進(jìn)行測序，對分離物進(jìn)行分子鑒定。

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