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Reverse Taxonomy for Elucidating Diversity of Insect-Associated Nematodes: A Case Study with Termites

機(jī)譯:反向分類昆蟲線蟲相關(guān)的闡發(fā)多樣性的個(gè)案研究與白蟻

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摘要

BackgroundThe molecular operational taxonomic unit (MOTU) has recently been applied to microbial and microscopic animal biodiversity surveys. However, in many cases, some of the MOTUs cannot be definitively tied to any of the taxonomic groups in current databases. To surmount these limitations, the concept of “reverse taxonomy” has been proposed, i.e. to primarily list the MOTUs with morphological information, and then identify and/or describe them at genus/species level using subsamples or by re-isolating the target organisms. Nevertheless, the application of “reverse taxonomy” has not been sufficiently evaluated. Therefore, the practical applicability of “reverse taxonomy” is tested using termite-associated nematodes as a model system for phoretic/parasitic organisms which have high habitat specificity and a potential handle (their termite host species) for re-isolation attempts.
機(jī)譯:背景技術(shù)分子操作分類單位(MOTU)最近已用于微生物和微觀動(dòng)物生物多樣性調(diào)查。但是,在許多情況下,某些MOTU不能確定地與當(dāng)前數(shù)據(jù)庫(kù)中的任何分類學(xué)類別相關(guān)聯(lián)。為了克服這些限制,提出了“反向分類法”的概念,即主要列出具有形態(tài)學(xué)信息的MOTU,然后使用子樣本或通過重新分離目標(biāo)生物在屬/種水平上識(shí)別和/或描述它們。但是,“反向分類法”的應(yīng)用尚未得到充分評(píng)估。因此,使用與白蟻相關(guān)的線蟲作為隱棲/寄生生物的模型系統(tǒng)來(lái)測(cè)試“反向分類法”的實(shí)際適用性,隱喻/寄生生物具有較高的棲息地特異性,并且具有潛在的柄(其白蟻宿主物種)進(jìn)行重新分離的嘗試。

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