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首頁> 美國衛(wèi)生研究院文獻>Journal of Clinical Microbiology >Competitive enzyme immunoassay for antibodies to a 43000-molecular-weight Francisella tularensis outer membrane protein for the diagnosis of tularemia.
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Competitive enzyme immunoassay for antibodies to a 43000-molecular-weight Francisella tularensis outer membrane protein for the diagnosis of tularemia.

機譯：競爭性酶免疫分析法檢測43000分子量的土拉弗朗西斯菌外膜蛋白的抗體以診斷Tularemia。

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Antibodies against a 43,000-molecular-weight Francisella tularensis outer membrane (OM) protein (43K protein) were measured in paired serum specimens from 23 patients with tularemia and matched against antibodies in sera from 25 patients with nontularemic infectious diseases and from 25 blood donors. Antibodies were measured by a competition enzyme-linked immunosorbent assay which tested the ability of human serum to compete with rabbit anti-43K protein antibodies for its binding to the F. tularensis OM coat. The sera from nontularemic patients and from blood donors, in dilutions of 1:16 and 1:64, respectively, showed no or very low levels of antibodies. All of the tularemia patients showed positive tests with the first, the second, or both of the serum specimens examined. For instance, with serum diluted 1:64, each of the serum specimens showed a sensitivity of 95.7% and a specificity of 96%. When used for antibody competition in Western blotting (immunoblotting), the rabbit anti-43K selectivity blocked the binding of human serum antibodies to the 43,000-molecular-weight protein. This protein was immunoaccessible in Formalin-killed F. tularensis. These data indicate an important role of the 43,000-molecular-weight OM protein in the immunobiology of tularemia and emphasize its potential usefulness as an antigen in serodiagnostic tests.
機譯：在來自23例圖拉血病患者的配對血清樣本中測量了針對43,000分子量的土拉弗朗西斯菌外膜（OM）蛋白（43K蛋白）的抗體，并與25例非圖萊姆傳染病患者和25個獻血者的血清中的抗體相匹配。通過競爭酶聯(lián)免疫吸附測定法測量抗體，該測定法測試了人血清與兔抗43K蛋白抗體競爭與土拉弗雷德氏菌OM外套結(jié)合的能力。來自非規(guī)管性患者和獻血者的血清分別以1:16和1:64的稀釋度顯示無抗體或抗體水平很低。所有的tularemia患者在檢查的第一個，第二個或兩個血清樣本中均顯示陽性測試。例如，用1:64稀釋的血清，每個血清標(biāo)本的靈敏度為95.7％，特異性為96％。當(dāng)用于Western印跡（免疫印跡）中的抗體競爭時，兔抗43K選擇性阻斷了人血清抗體與43,000分子量蛋白的結(jié)合。該蛋白在福爾馬林殺死的圖萊特菌中是免疫可及的。這些數(shù)據(jù)表明，分子量為43,000的OM蛋白在Tularemia的免疫生物學(xué)中具有重要作用，并強調(diào)了其在血清學(xué)診斷測試中作為抗原的潛在用途。

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