国产bbaaaaa片,成年美女黄网站色视频免费,成年黄大片,а天堂中文最新一区二区三区,成人精品视频一区二区三区尤物

首頁> 美國衛(wèi)生研究院文獻(xiàn)>ACS Omega >Highly Sensitive and Selective Direct Detection ofZika Virus Particles in HumanBodily Fluids for Accurate Early Diagnosis of Infection
【2h】

Highly Sensitive and Selective Direct Detection ofZika Virus Particles in HumanBodily Fluids for Accurate Early Diagnosis of Infection

機(jī)譯：高度靈敏和選擇性的直接檢測人的寨卡病毒顆粒體液可對感染進(jìn)行準(zhǔn)確的早期診斷

代理獲取
本網(wǎng)站僅為用戶提供外文OA文獻(xiàn)查詢和代理獲取服務(wù)，本網(wǎng)站沒有原文。下單后我們將采用程序或人工為您竭誠獲取高質(zhì)量的原文，但由于OA文獻(xiàn)來源多樣且變更頻繁，仍可能出現(xiàn)獲取不到、文獻(xiàn)不完整或與標(biāo)題不符等情況，如果獲取不到我們將提供退款服務(wù)。請知悉。
  • 摘要
  • 著錄項(xiàng)
  • 相似文獻(xiàn)
  • 相關(guān)主題

摘要

Zika virus (ZIKV) is an arbovirus that caused widespread panic beginning in 2015 in northeastern Brazil due to the threatening link between infection and fetal abnormalities such as microcephaly, spontaneous abortions, and stillbirths. Since the epidemic began, the virus has been further investigated, unveiling that the long-term dangers of ZIKV infection go beyond fetal neurological impairment. Characterization of the active infection has proven difficult as only 20% of infected individuals are symptomatic. Additionally, ZIKV is often misdiagnosed due to serological cross-reactivity with similar flaviviruses such as dengue, yellow fever, and West Nile. To date, there is no approved vaccine or therapy against ZIKV, highlighting the urgent need to accurately identify active infection to help minimize the spread of the virus. Herein, we describe a highly specific and sensitive enzyme-linked immunosorbent assay to detect early active ZIKV using neutralizing human monoclonal antibodies isolated from infected patients in Brazil that do not cross-react with dengue viruses 1–4 and bind directlyto a ZIKV immunodominant epitope. The calculated limits of detectionof active ZIKV fall within the physiological ranges of the virus inhuman bodily fluids. This selective immunoassay creates the platformrequired for future translation toward a point-of-care assay for ZIKV,a necessity to diagnose active ZIKV in the remote regions of whichit thrives.
機(jī)譯：Zika病毒（ZIKV）是一種蟲媒病毒，由于感染與胎兒畸形（例如小頭畸形，自然流產(chǎn)和死胎）之間的威脅性聯(lián)系，從2015年開始在巴西東北部引起廣泛恐慌。自流行開始以來，對該病毒進(jìn)行了進(jìn)一步的研究，發(fā)現(xiàn)ZIKV感染的長期危險(xiǎn)已超出胎兒神經(jīng)系統(tǒng)損害。由于僅20％的受感染個(gè)體是有癥狀的，因此很難確定活動性感染的特征。此外，由于與類似黃病毒（如登革熱，黃熱病和西尼羅河病毒）的血清學(xué)交叉反應(yīng)，經(jīng)常會誤診ZIKV。迄今為止，尚無針對ZIKV的批準(zhǔn)的疫苗或療法，突顯了迫切需要準(zhǔn)確識別主動感染以幫助最大程度地減少病毒傳播。本文中，我們描述了一種高度特異性和靈敏的酶聯(lián)免疫吸附測定法，可使用中和人單克隆抗體檢測早期活躍的ZIKV，該中和人單克隆抗體是從巴西受感染患者中分離的，不會與登革熱病毒1-4交叉反應(yīng)并直接結(jié)合ZIKV免疫優(yōu)勢表位。計(jì)算出的檢出限的ZIKV活性屬于病毒的生理范圍。人的體液。這種選擇性免疫測定創(chuàng)造了平臺將來需要轉(zhuǎn)換為ZIKV的即時(shí)檢驗(yàn)，在其偏遠(yuǎn)地區(qū)診斷活動ZIKV的必要性它蓬勃發(fā)展。

著錄項(xiàng)

相似文獻(xiàn)

  • 外文文獻(xiàn)
  • 中文文獻(xiàn)
  • 專利
代理獲取

客服郵箱：kefu@zhangqiaokeyan.com

京公網(wǎng)安備：11010802029741號 ICP備案號：京ICP備15016152號-6 六維聯(lián)合信息科技 (北京) 有限公司?版權(quán)所有

  • 客服微信

  • 服務(wù)號