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Beta-N-methylamino-l-alanine: LC-MS/MS Optimization Screening of Cyanobacterial Strains and Occurrence in Shellfish from Thau a French Mediterranean Lagoon

機(jī)譯：β-N-甲基氨基-1-丙氨酸：LC-MS / MS優(yōu)化藍(lán)藻菌株的篩選以及法國地中海瀉湖Thau的貝類中的出現(xiàn)

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β-N-methylamino-l-alanine (BMAA) is a neurotoxic non-protein amino acid suggested to be involved in neurodegenerative diseases. It was reported to be produced by cyanobacteria, but also found in edible aquatic organisms, thus raising concern of a widespread human exposure. However, the chemical analysis of BMAA and its isomers are controversial, mainly due to the lack of selectivity of the analytical methods. Using factorial design, we have optimized the chromatographic separation of underivatized analogues by a hydrophilic interaction chromatography coupled to tandem mass spectrometry (HILIC-MS/MS) method. A combination of an effective solid phase extraction (SPE) clean-up, appropriate chromatographic resolution and the use of specific mass spectral transitions allowed for the development of a highly selective and sensitive analytical procedure to identify and quantify BMAA and its isomers (in both free and total form) in cyanobacteria and mollusk matrices (LOQ of 0.225 and 0.15 µg/g dry weight, respectively). Ten species of cyanobacteria (six are reported to be BMAA producers) were screened with this method, and neither free nor bound BMAA could be found, while both free and bound DAB were present in almost all samples. Mussels and oysters collected in 2009 in the Thau Lagoon, France, were also screened, and bound BMAA and its two isomers, DAB and AEG, were observed in all samples (from 0.6 to 14.4 µg/g DW), while only several samples contained quantifiable free BMAA.
機(jī)譯：β-N-甲基氨基-1-丙氨酸（BMAA）是一種神經(jīng)毒性的非蛋白質(zhì)氨基酸，被認(rèn)為與神經(jīng)退行性疾病有關(guān)。據(jù)報(bào)道，它是由藍(lán)細(xì)菌產(chǎn)生的，但也存在于可食用的水生生物中，因此引起人們廣泛暴露于人類的擔(dān)憂。然而，主要由于缺乏分析方法的選擇性，對(duì)BMAA及其異構(gòu)體的化學(xué)分析存在爭議。使用析因設(shè)計(jì)，我們通過與串聯(lián)質(zhì)譜聯(lián)用的親水相互作用色譜法（HILIC-MS / MS）優(yōu)化了未衍生類似物的色譜分離。有效的固相萃?。⊿PE）凈化，適當(dāng)?shù)纳V分離度以及使用特定的質(zhì)譜躍遷的結(jié)合，可以開發(fā)出高度選擇性和靈敏的分析程序，以鑒定和定量BMAA及其異構(gòu)體（兩者均為游離和總形式）在藍(lán)細(xì)菌和軟體動(dòng)物基質(zhì)中（LOQ分別為干重0.225和0.15 μg / g）。用這種方法篩選了十種藍(lán)細(xì)菌（據(jù)報(bào)道六種是BMAA的生產(chǎn)者），既沒有發(fā)現(xiàn)游離的也沒有結(jié)合的BMAA，而幾乎所有樣品中都存在游離的和結(jié)合的DAB。還篩選了2009年在法國Thau瀉湖中收集的貽貝和牡蠣，并在所有樣品中觀察到了結(jié)合的BMAA及其兩種異構(gòu)體DAB和AEG（DW為0.6至14.4 μg / g），而僅包含幾個(gè)樣品可定量的免費(fèi)BMAA。

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