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首頁(yè)> 外文期刊>Journal of dairy science >Comparative analysis of the skim milk and milk fat globule membrane proteomes produced by Jersey cows grazing pastures with different plant species diversity
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Comparative analysis of the skim milk and milk fat globule membrane proteomes produced by Jersey cows grazing pastures with different plant species diversity

機(jī)譯：用不同植物物種多樣性放牧牧場(chǎng)生產(chǎn)的脫脂牛奶和乳脂肪球膜蛋白質(zhì)的比較分析

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摘要

The objective of this experiment was to identifyand characterize the bovine milk proteome within theskim milk fraction and milk fat globule membrane(MFGM)-associated fraction from 16 organically certifiedlactating Jersey cows after a short term of grazingpastures with or without annual forage crops (AFC).Cows were offered a partial mixed ration (～60% of drymatter intake) and approximately 40% of their totaldry matter intake as herbage. Eight cows were offereda cool-season grass–legume herbage (GLH), whichincluded orchardgrass (Dactylis glomerata), timothy(Phleum pratense), Kentucky bluegrass (Poa pratensis),and white clover (Trifolium repens). The other 8cows were offered the same GLH strip-tilled with theAFC, including oat (Avena sativa), millet (Pennisetumglaucum), teff (Eragrostis tef), buckwheat (Fagopyrumesculentum), and chickling vetch (Lathyrus sativus).Milk samples were collected from each cow during a.m.And p.m. milkings on d 19 to 21 of grazing, and compositemilk samples per cow were analyzed for (1) thehigh-abundance milk protein profile, (2) the skim milklow-abundance protein-enriched proteome, and (3) theMFGM proteome. Of the 443 proteins identified in theskim and MFGM proteomes, 433 were included in statisticalanalysis, including 68 proteins identified in theskim milk fraction and 365 in the MFGM-associatedfraction. Analysis of the skim and MFGM proteomesencompassed unique gene ontology profiles and proportionsof functional classifications. In response to diet,Αs1-casein as well as 8 low-abundance proteins werepresent in higher concentration or abundance in milkfrom cows grazing the GLH strip-tilled with the AFCcompared with milk from cows grazing GLH, suggestingthat even short-term grazing of pastures includingsome AFC may affect the milk proteome.
機(jī)譯：該實(shí)驗(yàn)的目的是識(shí)別并在牛奶中表征牛奶蛋白質(zhì)脫脂牛奶餾分和牛奶脂肪球膜（MFGM） - 從16個(gè)有機(jī)認(rèn)證的分?jǐn)?shù)在短期內(nèi)放牧后哺乳澤西母牛牧場(chǎng)有或沒(méi)有年度飼養(yǎng)作物（AFC）。奶牛被局部混合配給（?60％的干燥物質(zhì)攝入量）和總數(shù)的約40％干物質(zhì)攝入牧草。提供了八頭奶牛一個(gè)涼爽的草腿牧草（glh），哪個(gè)包括果園（Dactylis glomerata），蒂莫西（Phleum Praatense），肯塔基藍(lán)草（Poa pratensis），和白色三葉草（三粒repens）。其他8.奶牛提供了同樣的glh脫脂AFC，包括燕麥（Avena Sativa），小米（Pennisetumglaucum），teff（eragrostis tef），蕎麥（fagopyrumeSculentum）和鷹嘴豆（Lathyrus sativus）。在上午的每頭母牛中收集牛奶樣品。和下午放牧和復(fù)合材料的D 19至21塊分析每頭牛的牛奶樣品（1）高大豐牛奶蛋白質(zhì)概況，（2）脫脂牛奶低豐度蛋白質(zhì)富集的蛋白質(zhì)組，和（3）MFGM蛋白質(zhì)組。 443個(gè)蛋白質(zhì)中確定的脫脂和MFGM蛋白質(zhì)，433次統(tǒng)計(jì)分析，包括68個(gè)蛋白質(zhì)脫脂牛奶餾分和365在MFGM相關(guān)的分?jǐn)?shù)。脫脂和MFGM蛋白質(zhì)的分析包含獨(dú)特的基因本體概況和比例功能分類。為了回應(yīng)飲食，αS1-酪蛋白以及8個(gè)低豐度蛋白質(zhì)牛奶中濃度更高或豐富從奶牛放牧與亞足聯(lián)的glh脫脂與來(lái)自奶牛放牧的牛奶相比，提示甚至牧場(chǎng)的短期放牧包括一些AFC可能會(huì)影響牛奶蛋白質(zhì)組。

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  • 來(lái)源
    《Journal of dairy science》 |2020年第8期|7498-7508|共11頁(yè)
  • 作者單位

    Department of Animal and Veterinary Sciences The University of Vermont Burlington 05405;

    Vermont Genetics Network Proteomics Facility The University of Vermont Burlington 05405;

    Department of Animal and Veterinary Sciences The University of Vermont Burlington 05405;

    Department of Animal and Veterinary Sciences The University of Vermont Burlington 05405;

    Department of Animal and Veterinary Sciences The University of Vermont Burlington 05405 Department of Animal Sciences University ofFlorida Gainesville 32608;

    Department of Animal and Veterinary Sciences The University of Vermont Burlington 05405;

    Department of Agriculture Nutrition and Food Systems The University of New Hampshire Durham 03824;

    Department of Animal and Veterinary Sciences The University of Vermont Burlington 05405;

  • 收錄信息 美國(guó)《科學(xué)引文索引》(SCI);美國(guó)《生物學(xué)醫(yī)學(xué)文摘》(MEDLINE);美國(guó)《化學(xué)文摘》(CA);
  • 原文格式 PDF
  • 正文語(yǔ)種 eng
  • 中圖分類
  • 關(guān)鍵詞

    alternative forage crop; milk proteomics; liquid chromatography–tandem mass spectrometry; gene ontology;

    機(jī)譯：替代飼料作物;牛奶蛋白質(zhì)組學(xué);液相色譜 - 串聯(lián)質(zhì)譜;基因本體論;

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