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首頁> 外軍國防科技報(bào)告 >Effect of H2O2-Mediated Endophytic Fungal Elicitors on Essential Oil Accumulation in Suspension Cells of Cinnamomum longepaniculatum
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Effect of H2O2-Mediated Endophytic Fungal Elicitors on Essential Oil Accumulation in Suspension Cells of Cinnamomum longepaniculatum

機(jī)譯:H2O2介導(dǎo)的內(nèi)生真菌誘導(dǎo)劑對長壽肉桂懸浮細(xì)胞中精油積累的影響

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摘要

Hydrogen peroxide (H2O2) is a signal molecule that plays a crucial role in plant secondary metabolism. In order to explore the signaling mechanism of endophytic fungal elicitors (Penicillium commune 2J1) for promoting 1,8-eucalyptus accumulation in C. longepaniculatum, changes in the contents of H2O2 and 1,8-eucalyptus were investigated after the addition of elicitors to the C. longepaniculatum cultures. The experimental results showed that the 1,8-eucalyptus contents in C. longepaniculatum cells were increased upon addition of the endophytic fungal elicitors into the culture. It’s maybe through Hydrogen peroxide (H2O2) signal molecule. When different concentrations of elicitors were added to C. longepaniculatum suspension cells, the changes in the content of H2O2 and 1,8-eucalyptus showed the same trend, and both reached the highest value at 40 mg/L of elicitor concentration, indicating that the endogenous fungal elicitors affect the accumulation of 1,8-eucalyptus through H2O2 signaling molecular pathway. When CAT was added, the 1,8-eucalyptus decreased but was not completely inhibited, indicating that the elicitors also have other signaling pathways.,
機(jī)譯:過氧化氫(H2O2)是一種信號分子,在植物的次生代謝中起著至關(guān)重要的作用。為了探索內(nèi)生真菌激發(fā)子(Penicillium commune 2J1)促進(jìn)長脊孢菌中1,8-桉樹積累的信號傳導(dǎo)機(jī)制,在將激發(fā)子添加到C. longepaniculatum中后,研究了H2O2和1,8-桉樹的含量變化。 C. longepaniculatum培養(yǎng)物。實(shí)驗(yàn)結(jié)果表明,在培養(yǎng)物中加入內(nèi)生真菌激發(fā)子后,長鏈念珠菌細(xì)胞中1,8-桉樹的含量增加??赡苁峭ㄟ^過氧化氫(H2O2)信號分子。當(dāng)將不同濃度的激發(fā)子添加到長鏈念珠菌懸浮細(xì)胞中時(shí),H2O2和1,8-桉樹油的含量變化呈現(xiàn)出相同的趨勢,并且在40 mg / L激發(fā)子濃度下均達(dá)到最高值,表明內(nèi)源性真菌激發(fā)子通過H2O2信號分子途徑影響1,8-桉樹的積累。當(dāng)添加CAT時(shí),1,8-桉樹減少但未被完全抑制,表明引發(fā)子也具有其他信號通路。

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