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Characterization of Microscale Particles Using a Microfluidic Flow Cytometer Equipped With a Multi-Plex Photon Counter

機(jī)譯：使用配備了多重光子計(jì)數(shù)器的微流式細(xì)胞儀對(duì)微米級(jí)顆粒進(jìn)行表征

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摘要

We have shown the design and fabrication of a microfluidic flow cytometer. The microfluidic flow cytometer has been used to characterize microspheres of different sizes. The device is consisted of a microchannel, electronics, and integrated optics. The microchannel has three inlets. Two inlets are used to introduce sheath flows and one middle inlet is assigned as sample inlet. The sample flow is hydrodynamically focused at the center of the microchannel by two side streams (sheath flows). Also arrays of four chevron grooves compress the sample flow from the top and bottom of the microchannel. The core flow contains microspheres at a certain concentration. Detection of the microspheres at the interrogation region of the channel is performed by integrated optics and electronics. The scattered light emitted from the microspheres is collected by a multi-plex photo diode (MPPC). The results are recorded using data acquisition (DAQ) unit. The MPPCs employed in the setup is the new generation of photon counter devices with an excellent detection limit, a compact size, and capability of recording data at high gain compared to previous generation of photodetectors such as photomultipliers or avalanche photon diodes. The flow cytometer was sensitive enough to collect data from 3 μm microspheres using such mentioned sensitive photon counting unit. We have also used COMSOL Multiphysics software to investigate velocity and pressure distribution as well as concentration distribution along the microchannel. The average voltage collected by MPPC was 1.9 V for 10.2 μm and 1.6 V for 3.2 μm microsphere.
機(jī)譯：我們已經(jīng)展示了微流式細(xì)胞儀的設(shè)計(jì)和制造。微流式細(xì)胞儀已用于表征不同大小的微球。該設(shè)備由微通道，電子設(shè)備和集成光學(xué)器件組成。微通道具有三個(gè)入口。兩個(gè)入口用于引入鞘流，一個(gè)中間入口指定為樣品入口。樣品流通過(guò)兩條旁流（鞘流）以流體動(dòng)力學(xué)方式集中在微通道的中心。四個(gè)人字形凹槽的陣列還壓縮了來(lái)自微通道頂部和底部的樣品流。核心流包含一定濃度的微球。在通道的詢問(wèn)區(qū)域?qū)ξ⑶虻臋z測(cè)由集成的光學(xué)和電子設(shè)備執(zhí)行。從微球發(fā)出的散射光被多層光電二極管（MPPC）收集。使用數(shù)據(jù)采集（DAQ）單元記錄結(jié)果。與上一代光電檢測(cè)器（例如光電倍增管或雪崩光電二極管）相比，該設(shè)置中使用的MPPC是新一代光子計(jì)數(shù)器設(shè)備，具有出色的檢測(cè)極限，緊湊的尺寸以及以高增益記錄數(shù)據(jù)的能力。流式細(xì)胞儀足夠靈敏，可以使用上述靈敏光子計(jì)數(shù)單元從3μm微球中收集數(shù)據(jù)。我們還使用COMSOL Multiphysics軟件研究了沿微通道的速度和壓力分布以及濃度分布。 MPPC收集的平均電壓對(duì)于微球10.2μm為1.9 V，對(duì)于微球3.2μm為1.6V。

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  • 作者

    Asrar Pouya; Hashemi Nicole N.;

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  • 年度 2013
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