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Multiplexed electrochemical immunosensor for label-free detection of cardiac markers using a carbon nanofiber array chip

機譯:使用碳納米纖維陣列芯片的無電化學檢測心臟標記的多路復(fù)用電化學免疫傳感器

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摘要

We present an electrochemical multianalyte or multiplexed immunosensor for simultaneous label free detection of cardiac markers panel, comprising of C-reactive protein, cardiac troponin-I and myoglobin. The multi electrode biosensor chip contains nine identical but electrically isolated microelectrodes arranged in a 3 x 3 array configuration. Each electrode contains carbon nanofiber nanoelectrodes grown vertically using plasma enhanced chemical vapor deposition. A hydrophobic photoresist layer, lithographically etched on the chip, exposes the electrodes and helps to selectively immobilize the antibody probes for the three target cardiac biomarkers using carbodiimide chemistry. The real-time label free detection of the three cardiac markers from a mixture is demonstrated with high sensitivity and selectivity. Detection in complex protein mixtures in human blood serum does not show any false positives from non-specific protein adsorption. The results show that the present sensor can serve as a miniaturized, low cost lab-on-a-chip system for the detection of various biomarkers in healthcare, environmental monitoring and security applications. Published by Elsevier B.V.
機譯:我們提出了一種電化學多分析物或多重免疫傳感器,用于同時進行無標記的心臟標志物檢測,包括C反應(yīng)蛋白,心肌肌鈣蛋白I和肌紅蛋白。多電極生物傳感器芯片包含以3 x 3陣列配置排列的九個相同但電隔離的微電極。每個電極都包含使用等離子增強化學氣相沉積垂直生長的碳納米纖維納米電極。在芯片上進行光刻蝕刻的疏水性光致抗蝕劑層使電極暴露,并有助于使用碳二亞胺化學選擇性地固定針對三種靶標心臟生物標記物的抗體探針。以高靈敏度和高選擇性證明了混合物中三種心臟標記物的實時無標記檢測。在人血清中檢測復(fù)雜的蛋白質(zhì)混合物不會顯示任何非特異性蛋白質(zhì)吸附引起的假陽性。結(jié)果表明,本傳感器可以用作小型,低成本的芯片實驗室實驗室系統(tǒng),用于檢測醫(yī)療保健,環(huán)境監(jiān)測和安全應(yīng)用中的各種生物標記。由Elsevier B.V.發(fā)布

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