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首頁(yè)> 外文OA文獻(xiàn) >A trnH-psbA barcode genotyping assay for the detection of common apricot (Prunus armeniaca L.) adulteration in almond (Prunus dulcis Mill.)
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A trnH-psbA barcode genotyping assay for the detection of common apricot (Prunus armeniaca L.) adulteration in almond (Prunus dulcis Mill.)

機(jī)譯:用于檢測(cè)杏仁(Prunus Dulcis Mill)的常見(jiàn)杏(Prunus Armeniaca L.)摻假的TRNH-PSBA條形碼基因分型測(cè)定法

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摘要

Almond kernels can be mixed with apricot (Prunus armeniaca L.) seeds while preparing certain almond-based products such as marzipan, without an identifiable alteration in the final product’s sensory attributes. Consequently, apricot kernels are the most commonly utilized almond substitutes for the economic adulteration of almonds. In the present work, the trnH-psbA intergenic spacer was evaluated as a potential PCR-based authenticity marker of almond. The barcode enabled the straightforward discrimination of almond and apricot in their blends, based on diagnostic length polymorphisms between the two closely related Prunus species. The proposed assay simultaneously amplifies and distinguishes almond- and apricot-specific trnH-psbA barcode fragments from mixed samples. The barcode size of almond segregates significantly from peach as well, which is a secondary source for the economic adulteration of almond kernels. The proposed trnH-psbA genotyping assay is easy and selective for the discrimination of almond from its potential adulterants when present as admixtures.
機(jī)譯:杏仁內(nèi)核可以與杏(牧群亞美尼亞島L.)種子混合,同時(shí)準(zhǔn)備某些基于杏仁的產(chǎn)品,如Marzipan,沒(méi)有最終產(chǎn)品的感官屬性的可識(shí)別改變。因此,杏仁是杏仁經(jīng)濟(jì)摻假的最常用的杏仁替代品。在本作工作中,TrnH-PSBA代苯基間隔物評(píng)估為杏仁的潛在的PCR型真實(shí)性標(biāo)記。條形碼使其在其混合中的杏仁和杏的直接辨別,基于兩個(gè)密切相關(guān)的氏氏種類(lèi)之間的診斷長(zhǎng)度多態(tài)性。所提出的測(cè)定同時(shí)放大并區(qū)分混合樣品的杏仁和杏特異性TrnH-PSBA條形碼片段。杏仁的條形碼大小從桃子中顯著分離,這也是杏仁內(nèi)核經(jīng)濟(jì)摻假的二級(jí)來(lái)源。所提出的TRNH-PSBA基因分型測(cè)定易于和選擇性地為杏仁從其潛在的摻雜劑鑒別作為混合物時(shí)。

著錄項(xiàng)

  • 作者

    Ayse Ozgur Uncu;

  • 作者單位
  • 年度 2020
  • 總頁(yè)數(shù)
  • 原文格式 PDF
  • 正文語(yǔ)種 esl/spa;eng
  • 中圖分類(lèi)

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